Coding

Part:BBa_K1405006:Design

Designed by: Xuan Wang,Feng Jing,Ningze Sun   Group: iGEM14_BNU-China   (2014-10-12)

INPN+YFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 429
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1882
    Illegal SapI.rc site found at 238


Design Notes

we found that using INP derivatives containing only N-domain (INPN) as display system successfully is possible. The passenger proteins are also various, such as Japanese Encephalitis Virus Pathogenicity, phosphate-binding protein and so on. But INP is outer membrane protein, we have no idea whether INP is located on menbrane successfully. So we want to fuse INP and YFP. We,lat least, can know INP is expressed or not by observing light of YFP.

Source

The sequence of inaK can be found in the The National Center for Biotechnology Information advances science and health by providing access to biomedical and genomic information(NCBI) and the gene was synthesized by a company.

References